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Main Profile

Full Name and Degrees: 
John P. Cooke, M.D., Ph.D.
Member Role: 
Lead Principal Investigator
Institutional affiliation: 
The Houston Methodist Research Institute

Dr. John Cooke joins the Consortium from Stanford University where he is Professor of Medicine and Associate Director of the Stanford Cardiovascular Institute.

He received his Ph.D. in physiology at the Mayo Graduate School of Medicine (1985).

Dr. Cooke has authored or co-authored over 350 full-length manuscripts. He also has authored or co-authored five books and co-authored 19 patents.

He has received several honors and awards including:

  • Master of the Society for Vascular Medicine, (2009)
  • Established Investigator Award, American Heart Association (1995)
  • Teaching Award, Dept of Medicine (2001)
  • "Best Doctors in the Bay Area", San Francisco Magazine (2003,2005)
  • President, Society for Vascular Medicine (2005-2007)
Hub Site: 
10-John Cooke

Dr. John Cooke (The Houston Methodist Research Institute) and Dr. Alan Friedman (Johns Hopkins University) will focus on the safe reprogramming and differentiation of adult cells to vascular and blood-forming cell lines for eventual application to blood or vascular disorders.

research focus: 

Dr. Cooke’s work focuses on translational work in vascular regeneration from molecule to man. The goal is to transfer basic research insights into clinical trials.

His lab’s mission is to build new blood vessels, reverse vascular senescence, and to improve vascular health, in a translational research effort that includes fundamental research and clinical trials.

The basic research is focused on induced pluripotential stem cells (iPSCs) for vascular regeneration. Dr. Cooke’s lab is developing cell-permeant proteins, and new chemical entities for nuclear reprogramming, for differentiation of iPSCs into endothelial cells.

Clinical research is focused on patients with peripheral arterial disease (PAD). 

  • Testing new therapies for angiogenesis and vascular regeneration.
    • novel small molecules
    • cell therapy
  • Ongoing trials include enhancing angiogenesis and increasing oxygen supply to the limbs of patients with PAD.
Recent publications: 
Wong WT, Huang NF, Botham CM, Sayed N, Cooke JP. Endothelial cells derived from nuclear reprogramming. Circ Res. 2012;111:1363-1375 Lee J, Sayed N, Hunter A, Au KF, Wong WH, Mocarski ES, Pera RR, Yakubov E, Cooke JP. Activation of innate immunity is required for efficient nuclear reprogramming. Cell. 2012;151:547-558 White MP, Rufaihah AJ, Liu L, Ghebremariam YT, Ivey KN, Cooke JP, Srivastava D. Limited gene expression variation in human embryonic stem cell and induced pluripotent stem cell derived endothelial cells. Stem Cells. 2012 Lai ES, Huang NF, Cooke JP, Fuller GG. Aligned nanofibrillar collagen regulates endothelial organization and migration. Regenerative medicine. 2012;7:649-661 Huang NF, Okogbaa J, Babakhanyan A, Cooke JP. Bioluminescence imaging of stem cell-based therapeutics for vascular regeneration. Theranostics. 2012;2:346-354 Volz KS, Miljan E, Khoo A, Cooke JP. Development of pluripotent stem cells for vascular therapy. Vascular pharmacology. 2012;56:288-296 Rufaihah AJ, Huang NF, Jame S, Lee JC, Nguyen HN, Byers B, De A, Okogbaa J, Rollins M, Reijo-Pera R, Gambhir SS, Cooke JP. Endothelial cells derived from human iPSCs increase capillary density and improve perfusion in a mouse model of peripheral arterial disease. Arterioscler Thromb Vasc Biol. 2011;31:e72-79 Yang WC, Welsh JP, Lee J, Cooke JP, Swartz JR. Solubility partner if2 domain i enables high yield synthesis of transducible transcription factors in escherichia coli. Protein Expr Purif. 2011;80:145-151 Chang E, Forsberg EC, Wu J, Bingyin W, Prohaska SS, Allsopp R, Weissman IL, Cooke JP. Cholinergic activation of hematopoietic stem cells: Role in tobacco-related disease? Vasc Med. 2010;15:375-385 Leeper NJ, Hunter AL, Cooke JP. Stem cell therapy for vascular regeneration: Adult, embryonic, and induced pluripotent stem cells. Circulation. 2010;122:517-526 Huang NF, Patlolla B, Abilez O, Sharma H, Rajadas J, Beygui RE, Zarins CK, Cooke JP. A matrix micropatterning platform for cell localization and stem cell fate determination. Acta Biomater. 2010;6:4614-4621 Huang NF, Niiyama H, Peter C, De A, Natkunam Y, Fleissner F, Li Z, Rollins MD, Wu JC, Gambhir SS, Cooke JP. Embryonic stem cell-derived endothelial cells engraft into the ischemic hindlimb and restore perfusion. Arterioscler Thromb Vasc Biol. 2010;30:984-991 Huang NF, Fleissner F, Sun J, Cooke JP. Role of nitric oxide signaling in endothelial differentiation of embryonic stem cells. Stem Cells Dev. 2010;19:1617-1626 Embryonic stem cell-derived endothelial cells for treatment of hindlimb ischemia. Huang NF, Niiyama H, De A, Gambhir SS, Cooke JP. J Vis Exp. 2009; (23) http://www.unboundmedicine.com/medline/ebm/record/19229180/abstract Cholinergic modulation of angiogenesis: role of the 7 nicotinic acetylcholine receptor. Wu JC, Chruscinski A, De Jesus Perez VA, Singh H, Pitsiouni M, Rabinovitch M, Utz PJ, Cooke JP. J Cell Biochem. 2009; 108 (2): 433-46 http://www3.interscience.wiley.com/journal/122518359/abstract Cell-free production of transducible transcription factors for nuclear reprogramming. Yang WC, Patel KG, Lee J, Ghebremariam YT, Wong HE, Cooke JP, Swartz JR. Biotechnol Bioeng. 2009; 104 (6): 1047-58 http://www3.interscience.wiley.com/journal/122577812/abstract
6670 Bertner St MS Houston, TX 77030
Profile Page Photo: 
John P. Cooke, M.D., Ph.D.

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