PCBC Multiplexed in situ Hybridization Workshop (Materials and Recordings)

With the support of the NHLBI Progenitor Cell Biology Consortium (PCBC), Mark Krasnow, Tushar Desai, Pehr Harbury, and colleagues hosted a “PCBC Multiplexed in situ Hybridization Workshop” on March 22, 2017, at Stanford University.

“Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling” – Morrisey Hub Site 14

Drs. Katherine McCauley, Finn Hawkins, Darrell Kotton, and colleagues report that cyclical modulation of the canonical Wnt signaling pathway enables rapid directed differentiation of human iPSCs via an NKX2-1+ progenitor intermediate into functional proximal airway organoids. [30Mar2017 Cell Stem Cell]


Link to full article:



Protocols and Reagents

Platform for Sharing and Accessing Available Protocols and Reagents

The PCBC Synapse platform, in addition to storing data is a resource for the aggregation and dissemination of protocols and reagents for the consortium.


Lehanna Sanders, a graduate student in the Hatzopoulos Hub Site 11 has initiated this PCBC Blog. Other team memebers include Nicole Stone (Srivastava Hub Site 15) and Kaytlyn  Gerbin (Charles Murray's Lab - Morrisey Hub Site 14). To join the team, contact Ling Tang (ltang@epi.umaryland.edu) or Andrea Lefever (alefever@epi.umaryland.edu) for permission to post. For blog charter click here. 

Generation of multipotent induced cardiac progenitor cells from mouse fibroblasts and potency testing in ex vivo mouse embryos

Drs. Timothy Kamp, Pratik Lalit, Adriana Rodriguez, and Karen Downs share a protocol that generates induced cardiac progenitor cells (iCPCs) from mouse adult fibroblasts using forced expression of Mesp1, Tbx5, Gata4, Nkx2.5 and Baf60c (MTGNB) along with Wnt and JAK/STAT signaling. [20April2017 Nature Protocols]

Link to full article:


Job Opportunity: Assistant/Associate Project Scientist Position at UC Berkeley

Assistant/Associate Project Scientist Position at UC Berkeley

Recruitment Period:

Request for Applications (RFA): Pilot Projects for a Human Cell Atlas

The Human Cell Atlas is a global effort to create a reference map of all cells in the healthy human body as a resource for studies of health and disease.

The Chan Zuckerberg Initiative invites applications for one year pilot projects to develop technologies for the Human Cell Atlas, establish best practices in the field, and begin a common data archive for analysis and investigation.

PCBC Stem Cell Training at Stanford University, April 2017

With the support of the NHLBI Progenitor Cell Biology Consortium (PCBC), the Stanford Center for Human Embryonic Stem Cell Research and Education welcomes  both PCBC and PCTC graduate students, fellows, and faculty members to apply for one or both of the following training courses:

"Molecular, Phenotypic, and Sample-associated Data to Describe Pluripotent Stem Cell Lines and Derivatives"

Kenneth Daily, Shannan Sui, Lynn Schriml, and members of the PCBC bioinformatics committee report on the comprehensive characterization and differentiation of 57 pluripotent stem cell lines from 10 laboratories.  

Raw data, metadata, analysis and processing code, and documentation are publicly available at https://www.synapse.org/pcbc.

Click on the link below for full article from Scientific Data:


Publications from Dr. Jay Zhang and colleagues on cardiovascular tissue constructs


Jay Zhang and colleagues reported on the development and validation of 3D human cardiovascular tissue equivalents. Circulation Research (January 5th, 2017)

Click on the links below to access the review:

From Microscale Devices to 3D Printing: Advances in Fabrication of 3D Cardiovascular Tissues


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