“PAX7 Targets, CD54, Integrin α9β1, and SDC2, Allow Isolation of Human ESC/iPSC-Derived Myogenic Progenitors” – Thomson Hub Site 02

Drs. Alessandro Magli, James Thomson, Rita Perlingeiro, and colleagues identify CD54, integrin a9b1, and SDC2 as surface markers that enable the purification of myogenic progenitors endowed with long-term regenerative potential. [27Jun2017 Cell Reports]

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Specification and Diversification of Pericytes and Smooth Muscle Cells from Mesenchymoangioblasts

Drs. Akhilesh Kumar, James Thomson, Igor Slukvin, and colleagues find that mesodermal pericytes and smooth muscle cells in human pluripotent stem cell cultures originate from a common endothelial and mesenchymal cell precursor, the mesenchymoangioblast. They show how different lineages of mural cells are specified from mesenchymoangioblasts and define stage- and lineage-specific markers for vasculogenic cells. [30May2017 Cell Reports]

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FOAs: Revision Applications for Regenerative Medicine Innovation Projects (RMIP): R43/R44; R41/R42; U54; UM1; UC4; U24; R01; R24; UM2; P50; P41; U01

FOAs: Revision Applications for Regenerative Medicine Innovation Projects (RMIP) [R43/R44; R41/R42; U54; UM1; UC4; U24; R01; R24; UM2; P50; P41; U01].

PCBC Multiplexed in situ Hybridization Workshop (Materials and Recordings)

With the support of the NHLBI Progenitor Cell Biology Consortium (PCBC), Mark Krasnow, Tushar Desai, Pehr Harbury, and colleagues hosted a “PCBC Multiplexed in situ Hybridization Workshop” on March 22, 2017, at Stanford University.

“Efficient Derivation of Functional Human Airway Epithelium from Pluripotent Stem Cells via Temporal Regulation of Wnt Signaling” – Morrisey Hub Site 14

Drs. Katherine McCauley, Finn Hawkins, Darrell Kotton, and colleagues report that cyclical modulation of the canonical Wnt signaling pathway enables rapid directed differentiation of human iPSCs via an NKX2-1+ progenitor intermediate into functional proximal airway organoids. [30Mar2017 Cell Stem Cell]


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Protocols and Reagents

Platform for Sharing and Accessing Available Protocols and Reagents

The PCBC Synapse platform, in addition to storing data is a resource for the aggregation and dissemination of protocols and reagents for the consortium.


Lehanna Sanders, a graduate student in the Hatzopoulos Hub Site 11 has initiated this PCBC Blog. Other team memebers include Nicole Stone (Srivastava Hub Site 15) and Kaytlyn  Gerbin (Charles Murray's Lab - Morrisey Hub Site 14). To join the team, contact Ling Tang (ltang@epi.umaryland.edu) or Andrea Lefever (alefever@epi.umaryland.edu) for permission to post. For blog charter click here. 

Generation of multipotent induced cardiac progenitor cells from mouse fibroblasts and potency testing in ex vivo mouse embryos

Drs. Timothy Kamp, Pratik Lalit, Adriana Rodriguez, and Karen Downs share a protocol that generates induced cardiac progenitor cells (iCPCs) from mouse adult fibroblasts using forced expression of Mesp1, Tbx5, Gata4, Nkx2.5 and Baf60c (MTGNB) along with Wnt and JAK/STAT signaling. [20April2017 Nature Protocols]

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Job Opportunity: Assistant/Associate Project Scientist Position at UC Berkeley

Assistant/Associate Project Scientist Position at UC Berkeley

Recruitment Period:

Request for Applications (RFA): Pilot Projects for a Human Cell Atlas

The Human Cell Atlas is a global effort to create a reference map of all cells in the healthy human body as a resource for studies of health and disease.

The Chan Zuckerberg Initiative invites applications for one year pilot projects to develop technologies for the Human Cell Atlas, establish best practices in the field, and begin a common data archive for analysis and investigation.


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