Protocols and Reagents
Platform for Sharing and Accessing Available Protocols and Reagents
The PCBC Synapse platform, in addition to storing data is a resource for the aggregation and dissemination of protocols and reagents for the consortium.
Currently, we have a list of shared protocols with corresponding reagents in Synapse for your access. This includes protocols for reprogramming and cell cultures (e.g., episomal reprogramming and 3D cell cultures). Click here and login to Synapse to access the protocol collections.
If you need a Synapse account, please register for one.
If you have a protocol that you would like to submit for sharing, please click here to use the submission form.
If you have reagents that you would like to submit for sharing, please click here to use the submission form.
Below is a list of protocols and reagents in Synapse:
|
Source |
Protocol/Reagent |
ID |
|---|---|---|
|
Cooke Hub Site 10 |
3D Matrigel Culture Protocol |
|
|
Metzger Laboratory (Garry Hub Site 16) |
Adult Mouse Cardiac Myocyte Isolation |
|
|
Metzger Laboratory (Garry Hub Site 16) |
Adult Rat Cardiac Myocyte Isolation |
|
|
Cell Characterization Core |
C4-06 v01 Cryopreservation of hESC from mTeSR1 |
|
|
Cell Characterization Core |
C4-08 v01 Passage of Pluripotent Stem Cells in mTeSR1 |
|
|
Cell Characterization Core |
C4-09 v01 Thawing and Plating Pluripotent Stem Cells in mTeSR1 |
|
|
Cell Characterization Core |
C4-10 v01 Embryoid Body Protocol |
|
|
Cell Characterization Core |
C4-11 v01 Mesoderm Differentiation Protocol |
|
|
Cell Characterization Core |
C4-12 v01 Definitive Endoderm Differentiation Protocol |
|
|
Cell Characterization Core |
C4-13 v01 Ectoderm Differentiation Protocol |
|
|
Cell Characterization Core |
C4-14 v01 Teratoma Formation Protocol |
|
|
Bernstein Hub Site 09 |
Coating Plates with Notch Ligands |
|
|
Igor Slukvin Laboratory |
Completely Defined 2D System for the Differentiation of Human Pluripotent Stem Cells into Hematoendothelial Lineages |
|
|
Bernstein Hub Site 09 |
Cord blood expansion protocols |
|
|
Kevin D'Amour |
DAmour_Def_Endo_NatBiotech_2005.pdf |
|
|
Sunny Chan and Michael Kyba (Garry Hub Site 16) |
EB differentiation swirling protocol |
|
|
Kevin Eggan |
Eggan_iPSC_Neural Dif_NatBiotech_2011.pdf |
|
|
Garry Hub Site 16 |
Embryo dissection and FACS sorting |
|
|
Mervin Yoder |
Endothelial Differentiation and Analysis |
|
|
Metzger Laboratory (Garry Hub Site 16) |
Explanted human heart Cardiac Myocyte Isolation |
|
|
Garry Hub Site 16 |
FACS staining protocol for cardiac TroponinT for cardiomyocytes differentiated from iPS cells |
|
|
The Fred Hutch Cancer Institute (Torok-Storb Hub Site 05) |
FHCRC episomal reprogramming protocol |
|
|
The Fred Hutch Cancer Institute (Torok-Storb Hub Site 05) |
FHCRC retrovirus reprogramming protocol |
|
|
Zambidis Laboratory (Friedman Hub Site 03) |
Generation of Multipotent CD34+CD45+ Hematopoietic Progenitors from Human Induced Pluripotent Stem Cells |
|
|
Zambidis Laboratory (Friedman Hub Site 03) |
Growing Undifferentiated hESC and iPSC (Zambidis Lab Protocol) |
|
|
Qiang Xiong and Jay Zhang |
In vivo mapping of myocardial energetics |
|
|
Qiang Xiong and Jay Zhang |
In vivo measurement of myocardial ATP turnover |
|
|
Sunny Chan and Michael Kyba (Garry Hub Site 16) |
Intracellular staining for FACS analysis |
|
|
Luciene Borges and Rita Perlingeiro |
Isolation of Early Hematopoietic Cells from E7.5 embryos |
|
|
Garry Hub Site 16 |
Isolation of iPSC-CM for SIRPA MACS purification |
|
|
Garry Hub Site 16 |
Ki67 immunostaining for iPS cells |
|
|
Garry Hub Site 16 |
List of verified antibodies |
|
|
Robin Schroll |
Lonza_ManualsProductInstructions_Clonetics_human_smooth_muscle_cell_systems |
|
|
Robin Schroll |
Lonza_ManualsProductInstructions_Instructions_-_Airway_Epithelial_Cell_Systems |
|
|
Robin Schroll |
Lonza_ManualsProductInstructions_Instructions_-_Human_Fibroblast_Cell_Systems |
|
|
Alessandra Magli and Rita Perlingeiro |
Nuclear and cytoplasmic extracts from mouse EBs |
|
|
Tara Rasmussen (Garry Hub Site 16) |
Prep of adult organs for FACS sorting |
|
|
Robin Schroll |
Promocell_Protocols_HCM |
|
|
Robin Schroll |
Promocell_Protocols |
|
|
Jonathan Slack and James Dutton (Garry Hub Site 16) |
Slack and Dutton Lab human iPSC Protocols |
|
|
Su-Chan Zhang |
Zhang_Neural Diff_hiPSC_PNAS_2010.pdf |
|
|
Robin Schroll |
gentleMACS-Total-RNA |
|
|
Robin Schroll |
trizol_reagent |
Copyright ©2013 NHLBI Progenitor Cell Biology Consortium.
