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Protocols and Reagents

Platform for Sharing and Accessing Available Protocols and Reagents

The PCBC Synapse platform, in addition to storing data is a resource for the aggregation and dissemination of protocols and reagents for the consortium.

Currently, we have a list of shared protocols with corresponding reagents in Synapse for your access. This includes protocols for reprogramming and cell cultures (e.g., episomal reprogramming and 3D cell cultures). Click here and login to Synapse to access the protocol collections.

If you need a Synapse account, please register for one.

If you have a protocol that you would like to submit for sharing, please click here to use the submission form.

If you have reagents that you would like to submit for sharing, please click here to use the submission form.

Below is a list of protocols and reagents in Synapse:

Source

Protocol/Reagent

ID

Cooke Hub Site 10

3D Matrigel Culture Protocol

syn2872957

Metzger Laboratory

(Garry Hub Site 16)

Adult Mouse Cardiac Myocyte Isolation

syn3158111

Metzger Laboratory

(Garry Hub Site 16)

Adult Rat Cardiac Myocyte Isolation

syn3158113

Cell Characterization Core

C4-06 v01 Cryopreservation of hESC from mTeSR1

syn2724707

Cell Characterization Core

C4-08 v01 Passage of Pluripotent Stem Cells in mTeSR1

syn2724700

Cell Characterization Core

C4-09 v01 Thawing and Plating Pluripotent Stem Cells in mTeSR1

syn2724705

Cell Characterization Core

C4-10 v01 Embryoid Body Protocol

syn2512370

Cell Characterization Core

C4-11 v01 Mesoderm Differentiation Protocol

syn2512371

Cell Characterization Core

C4-12 v01 Definitive Endoderm Differentiation Protocol

syn2512372

Cell Characterization Core

C4-13 v01 Ectoderm Differentiation Protocol

syn2512373

Cell Characterization Core

C4-14 v01 Teratoma Formation Protocol

syn3103753

Bernstein Hub Site 09

Coating Plates with Notch Ligands

syn5842519

Igor Slukvin Laboratory

Completely Defined 2D System for the Differentiation of Human Pluripotent Stem Cells into Hematoendothelial Lineages

syn3232125

Bernstein Hub Site 09

Cord blood expansion protocols

syn5842518

Kevin D'Amour

DAmour_Def_Endo_NatBiotech_2005.pdf

syn2872959

Sunny Chan and Michael Kyba

(Garry Hub Site 16)

EB differentiation swirling protocol

syn3158101

Kevin Eggan

Eggan_iPSC_Neural Dif_NatBiotech_2011.pdf

syn2872960

Garry Hub Site 16

Embryo dissection and FACS sorting

syn3158103

Mervin Yoder

Endothelial Differentiation and Analysis

syn2872963

Metzger Laboratory

(Garry Hub Site 16)

Explanted human heart Cardiac Myocyte Isolation

syn3158105

Garry Hub Site 16

FACS staining protocol for cardiac TroponinT for cardiomyocytes differentiated from iPS cells

syn3158104

The Fred Hutch Cancer Institute

(Torok-Storb Hub Site 05)

FHCRC episomal reprogramming protocol

syn2534308

The Fred Hutch Cancer Institute

(Torok-Storb Hub Site 05)

FHCRC retrovirus reprogramming protocol

syn2534313

Zambidis Laboratory

(Friedman Hub Site 03)

Generation of Multipotent CD34+CD45+ Hematopoietic Progenitors from Human Induced Pluripotent Stem Cells

syn2872965

Zambidis Laboratory

(Friedman Hub Site 03)

Growing Undifferentiated hESC and iPSC (Zambidis Lab Protocol)

syn2872964

Qiang Xiong and Jay Zhang

In vivo mapping of myocardial energetics

syn3158110

Qiang Xiong and Jay Zhang

In vivo measurement of myocardial ATP turnover

syn3158094

Sunny Chan and Michael Kyba

(Garry Hub Site 16)

Intracellular staining for FACS analysis

syn3158106

Luciene Borges and Rita Perlingeiro

Isolation of Early Hematopoietic Cells from E7.5 embryos

syn3158107

Garry Hub Site 16

Isolation of iPSC-CM for SIRPA MACS purification

syn3158109

Garry Hub Site 16

Ki67 immunostaining for iPS cells

syn3158108

Garry Hub Site 16

List of verified antibodies

syn3158114

Robin Schroll

Lonza_ManualsProductInstructions_Clonetics_human_smooth_muscle_cell_systems

syn3506274

Robin Schroll

Lonza_ManualsProductInstructions_Instructions_-_Airway_Epithelial_Cell_Systems

syn3506272

Robin Schroll

Lonza_ManualsProductInstructions_Instructions_-_Human_Fibroblast_Cell_Systems

syn3506275

Alessandra Magli and Rita Perlingeiro

Nuclear and cytoplasmic extracts from mouse EBs

syn3158112

Tara Rasmussen

(Garry Hub Site 16)

Prep of adult organs for FACS sorting

syn3158093

Robin Schroll

Promocell_Protocols_HCM

syn3506282

Robin Schroll

Promocell_Protocols

syn3506284

Jonathan Slack and James Dutton

(Garry Hub Site 16)

Slack and Dutton Lab human iPSC Protocols

syn2872962

Su-Chan Zhang

Zhang_Neural Diff_hiPSC_PNAS_2010.pdf

syn2872966

Robin Schroll

gentleMACS-Total-RNA

syn3506262

Robin Schroll

trizol_reagent

syn3506283

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