HS10 - Cooke Hub Site

Dr. Cooke’s translational research program is focused on vascular regeneration.  The focus is on restoration or stimulation of endothelial functions such as vasodilation and angiogenesis, using small molecules or stem cell therapies.  In his 25 years of translational endothelial biology, he first described and characterized the anti-atherogenic effects of endothelium-derived nitric oxide; the anti-angiogenic effect of the NO synthase inhibitor ADMA; the angiogenic pathway mediated by endothelial nicotinic acetylcholine receptors; the role for this pathway in states of pathological angiogenesis; and developed an antagonist of the pathway that is now in Phase II clinical trials.  His clinical research group has explored the use of angiogenic agents and adult stem cells in the treatment of peripheral arterial disease.  More recently, he has generated and characterized endothelial cells derived from human iPSCs, and explored their role in angiogenesis and vascular regeneration. Recent insights from the laboratory have clarified the role of innate immune signaling in nuclear reprogramming to pluripotency and therapeutic transdifferentiation for vascular disease.

Protocols and Reagents that are associated with this hub site can be accessed by logging into Synapse and searching the database from the appropriate list of protocols or reagents.  Click here for instructions to access Synapse.

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Publication: 
Author(s) Title Link
Dunn, Louise L; Buckle, Andrew M; Cooke, John P; Ng, Martin K C The emerging role of the thioredoxin system in angiogenesis view
Chang, Edwin; Forsberg, E Camilla; Wu, Jenny; Bingyin Wang; Prohaska, Susan S; Allsopp, Rich; Weissman, Irving L; Cooke, John P Cholinergic activation of hematopoietic stem cells: role in tobacco-related disease? view
Leeper, Nicholas J; Hunter, Arwen L; Cooke, John P Stem cell therapy for vascular regeneration: adult, embryonic, and induced pluripotent stem cells view
Gilbert, P M; Havenstrite, K L; Magnusson, K E G; Sacco, A; Leonardi, N A; Kraft, P; Nguyen, N K; Thrun, S; Lutolf, M P; Blau, H M Substrate elasticity regulates skeletal muscle stem cell self-renewal in culture view
Pajcini, Kostandin V; Corbel, Stephane Y; Sage, Julien; Pomerantz, Jason H; Blau, Helen M Transient inactivation of Rb and ARF yields regenerative cells from postmitotic mammalian muscle view
Cooke, John P DDAH: a target for vascular therapy? view
Axtell, Andrea L; Gomari, Fatemeh A; Cooke, John P Assessing endothelial vasodilator function with the Endo-PAT 2000 view
• Nazish Sayed, Frank Ospino, Farhan Himmati, Jieun Lee, Palas Chanda, Edward S. Mocarski, John P. Cooke Retinoic Acid Inducible Gene 1 Protein (RIG1)-Like Receptor Pathway Is Required for Efficient Nuclear Reprogramming view

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